Developmental patterns of FASN and LIPE mRNA expression in adipose tissue of growing Jinhua and Landrace gilts
Miao, Z.,Henan Inst. of Science and Technology, XinXiang (China). Coll. of Animal Science | Zhu, F.,Zhejiang Univ., Hangzhou (China). Key Lab. for Molecular Animal Nutrition | Zhang, H.,Henan Inst. of Science and Technology, XinXiang (China). Coll. of Animal Science | Chang, X.,Henan Inst. of Science and Technology, XinXiang (China). Coll. of Animal Science | Xie, H.,Henan Inst. of Science and Technology, XinXiang (China). Coll. of Animal Science | Zhang, J.,Henan Inst. of Science and Technology, XinXiang (China). Coll. of Animal Science | Xu, Z.,Zhejiang Univ., Hangzhou (China). Key Lab. for Molecular Animal Nutrition
The present study was aimed to investigate the developmental patterns of fatty acid synthase (FASN) and lipase (LIPE, hormone-sensitive) mRNA in adipose tissue in pigs of different breeds and the relation with carcass fat content. Subcutaneous adipose tissue was sampled and total RNA was extracted to determine FASN and LIPE mRNA levels by semi-quantitative RT-PCR. The results showed that the FASN mRNA level increased with age (P less than 0.05) and Jinhua gilts expressed higher FASN mRNA compared with Landrace gilts at 80 and 125 days of age (P less than 0.05). In addition, Jinhua gilts expressed lower LIPE mRNA compared with Landrace gilts at 80 days of age (P less than 0.01). Furthermore, the ratio of FASN/LIPE mRNA had a similar model in the two breeds, and was higher in Jinhua gilts than that in Landrace gilts at 80 and 125 days of age (P less than 0.05). The FASN mRNA level was positively related to carcass fat content in Jinhua and Landrace gilts (r = 0.802, P = 0.01; r = 0.734, P = 0.02; respectively), and the ratio of FASN/LIPE expression exhibited significantly positively related carcass fat content (r = 0.804, P = 0.01; r = 0.749, P = 0.02; respectively).
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