Immunohistochemical detection of wheat protein in model samples
Rezacova-Lukaskova, Z.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Vegetabilnich Potravin a Rostlinne Produkce | Tremlova, B.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Vegetabilnich Potravin a Rostlinne Produkce | Pospiech, M.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Vegetabilnich Potravin a Rostlinne Produkce | Rencova, E.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) | Randulova, Z.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Vegetabilnich Potravin a Rostlinne Produkce
The study focused on the optimisation of immunohistochemical examination for gluten content detection in model samples (pork meat with wheat semi-smooth flour, pork meat with wheat protein edible vital). The best results were achieved with immunohistochemical method based on avidin-biotin complex utilising polyclonal antibodies diluted 1:1000. The results demonstrate that the utilisation of immunohistochemical method which can detect as little as 0.1% of the added wheat protein is more advantageous than the commonly used ELISA method which reliably proves wheat protein levels from 0.4% upwards.
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