Allelic variations at Dhn4 and Dhn7 are associated with frost tolerance in barley
Holkova, L.,Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi | Mikulkova, P.,Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi | Hrstkova, P.,Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi | Prasil, I.T.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Bradacova, M.,Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi | Prasilova, P.,Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Chloupek, O.,Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi
The sequences of the closely linked barley dehydrin genes Dhn4 and Dhn7 are both known to vary allelically. Here we associated allelic constitution at Dhn4/7 with frost sensitivity across a panel of 30 diverse barley varieties. The combined presence of a 6 bp insertion in exon1 of Dhn4 and a 30 bp deletion in exon1 of Dhn7 was restricted to six-rowed winter and intermediate varieties characterised by relatively higher frost tolerance (12 genotypes; LT50 from -14.2 deg C to -15.6 deg C). The alternative combination was present with one exception (six-rowed winter variety Alissa) only in spring and two-rowed winter varieties (17 genotypes; LT50 from -10.0 deg C to -14.3 deg C). The genetic linkage between Dhn4 and Dhn7 identified e.g. in Dictoo and Morex varieties was verified by segregation analysis of F2 plants from a cross between two genotypes carrying different allelic combinations of Dhn4 and Dhn7 genes (two-rowed spring variety Akcent x six-rowed winter variety Okal). The potential of the former allelic combination as a marker for enhanced frost tolerance was tested in a sample of F5 derivatives of a cross between the two-rowed winter type variety Monaco (Akcent allele combination) and the six-rowed winter type variety Okal. Plants with the Okal allele combination showed significantly higher frost tolerance than those with the alternative growth habit. The effect of ear type on frost tolerance was insignificant.
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