Effects of leaf soluble sugars content and net photosynthetic rate of quince donor shoots on subsequent morphogenesis in leaf explants
Mingozzi, M.,Universita di Pisa (Italy). Dipartimento di Coltivazione e Difesa delle Specie Legnose G. Scaramuzzi | Morini, S.,Universita di Pisa (Italy). Dipartimento di Coltivazione e Difesa delle Specie Legnose G. Scaramuzzi | Lucchesini, M.,Universita di Pisa (Italy). Dipartimento di Biologia delle Piante Agrarie | Mensuali-Sodi, A.,Scuola Superiore Sant' Anna di Studi Universitari e di Perfezionamento, Pisa (Italy)
The effects of different growth conditions (ventilated and closed vessels, medium with 0, 15 and 30 g/cubic dm sucrose) during proliferation of donor Cydonia oblonga shoots (stage I) on net photosynthetic rate and soluble sugars content were evaluated. In order to assess the influence of these physiological parameters on morphogenesis, leaf explants harvested from donor shoots were induced to form somatic embryos and adventitious roots under ventilated and closed Petri dishes (stage II). Natural ventilation and low sucrose contents (0-15 g/cubic dm) promoted the photosynthetic rate of quince shoots. Biomass accumulation was the highest in shoots cultured with 30 g/cubic dm sucrose in both vessel types and 15 g/cubic dm sucrose under natural ventilation. Increasing sucrose content in the medium induced greater accumulation of sucrose in leaf tissues of donor shoots. The level of reducing sugars was higher than that of sucrose, and it appeared to be higher in shoots cultured under natural ventilation compared to those in closed vessels. Somatic embryogenesis and root regeneration were influenced by stage I and II treatments. A significant correlation between sucrose content in the leaves of donor shoots and the number of somatic embryos regenerated was found, suggesting that identification of biochemical and physiological characteristics of donor shoots associated with increased regeneration ability might be helpful for improving morphogenesis in plant tissue culture.
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