Overexpression of a Ran GTPase homologous gene, FaRan from tall fescue, in transgenic Arabidopsis
Lue, S.H.,Liaocheng Univ. (China). School of Agriculture | Fan, Y.L.,Liaocheng Univ. (China). School of Agriculture | Jin, C.X.,Henan Normal Univ. (China). School of Chemistry and Environmental Sciences
We isolated and characterized a novel Ran GTPase homologous gene, FaRan from Festuca arundinacea. Reverse transcriptase polymerase chain reaction analysis indicated that FaRan is broadly expressed in old mature leaves, young leaves, plumules, stems, infloresence meristems, but at different levels. Transcript of FaRan is higher in young meristems than in old ones. Ectopic expression of FaRan resulted in increased number of axillary buds and reduced apical dominance in transgenic Arabidopsis plants. These results suggest that FaRan in F. arundinacea may be involved in the initiation of meristem and subsequent growth as well as development. In addition, it also suggests that FaRan can be used potentially to improve turfgrass quality.
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