The role of arbuscular myeorrhizae in the grow th and zinc uptake of wheat plant grown on acalcareous soil contaminated with zinc

The experiment was conducted to study the influence of various arbuscular mycorrhizal fungi(AMF) species as a bioremediation agent for soil contaminated with Zinc. Wheat plant( Triticum aesitivum) - Giza 168 was grown on a calcareous soil and supplemented with six Zn addition levels of 0, 2, 4,6,8 and 10 mM kg' soil in the form of ZnSQ4.7H02 . Four AM fungal inocula namely Clam us spp (mixed), Glomus intraradiaces, Glomus macrocarpium, and Glomus fasciculatum, the first one was isolated from contaminated soil and were applied to the soil. The plants were collected after 60 days from sowing. Mycorrhizal colonization rate, plant dry weight (DW), Zn concentrations and Zn uptake were determined and uptake efficiency, translocation efficiency and phytoextraction efficiency were calculated. The Glomus spp-treated plants had higher mycorrhizal colonization rates than other inoculation-treated plants. All mycorrhizal species increased shoot and root 1)W, Glomus spp (mixed) was more effective than the others. Mycorrhizal plants accumulated more zinc in roots, however appeared a large reductions in shoots. The use of AM fungal for phytoremediation of the contaminated soil lead to more absorption of zinc in plant. The comparisons of the four AM fungal species indicate that the AM fungal represented by Glomus spp (mixed) showed a beneficial effect in phytoremediation of Zn-contaminated soils. Pot experiment was carried out at the green house of Faculty of Agriculture (Saba bacha), Alexandria University. The experiment was conducted to study the influence of various arbuscular mycorrhizal fungi(AMF) species as a bioremediation agent for soil contaminated with Zinc. Wheat plant( Triticum aesitivum) - Giza 168 was grown on a calcareous soil and supplemented with six Zn addition levels of 0, 2, 4,6,8 and 10 mM kg' soil in the form of ZnSQ4.7H02 . Four AM fungal inocula namely Clam us spp (mixed), Glomus intraradiaces, Glomus macrocarpium, and Glomus fasciculatum, the first one was isolated from contaminated soil and were applied to the soil. The plants were collected after 60 days from sowing. Mycorrhizal colonization rate, plant dry weight (DW), Zn concentrations and Zn uptake were determined and uptake efficiency, translocation efficiency and phytoextraction efficiency were calculated. The Glomus spp-treated plants had higher mycorrhizal colonization rates than other inoculation-treated plants. All mycorrhizal species increased shoot and root 1)W, Glomus spp (mixed) was more effective than the others. Mycorrhizal plants accumulated more zinc in roots, however appeared a large reductions in shoots. The use of AM fungal for phytoremediation of the contaminated soil lead to more absorption of zinc in plant. The comparisons of the four AM fungal species indicate that the AM fungal represented by Glomus spp (mixed) showed a beneficial effect in phytoremediation of Zn-contaminated soils