Effect of some supporting type, iba cncenration and their interaction on rooting of in vitro regenerated micro-shoots of polygala myrifolia L.
2010
Sarhan, A.M.Z. | El-Maadawy, E.I. | Saadawy, F.M. | Noor-El-Deen, TM.
A laboratory experiment was carried out at the Tissue Culture Laboratory, Horticulture Research Institute, Agricultural Research Center, during 2008/2009 to induce rooting on the in vitro regenerated micro-shoots of Polygala myrtifolia L. on MS medium. These shoots were supported in the culture using four gelling agents (agar at 7.0 g/l, gelrite at 2.0 g/1 and agar+gelrite (at 3.5+1.0 g/l, respectively), in addition to a filter paper bridge on a liquid MS medium. The micro-shoots were presoaked in different IBA concentrations (0.0, 0.5, 1.0 and 1.5 ppm) before inoculation on a growth regulator-free 3/4 MS medium. Results indicate that rooting % and total chlorophyll content increased significantly in agar + gelrite solidified medium, while gelrite significantly increased number of roots/plantlet. IBA at 1.0 ppm significantly increased rooting %, the number of roots/plantlet, root length, total chlorophyll, indoles and total soluble sugars %. The combined effect of the supporting type and [BA concentration exerted a significant effect. Rooting % increased to the significantly highest record when using agar + gelrite in a medium free of IBA. Therefore, from an economical point of view, it can be recommended in the rooting stage to use agar + gelrite (at 3.5+1.0 g/1 MS medium, respectively) without presoaking in IBA solutions to obtain the highest rooting %.
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