Evolution of total bacteria and metanogenic arqueas population along with anaerobic co-digestion of pig slurry | Evolución de la población bacteriana total y metanogénica en el proceso de co-digestión anaerobia de purines de cerdos
2011
Seradj, A.R., Universitat de Lleida (España). Escola Tècnica Superior d'Enginyeria Agraria de Lleida | Morazan, H.J., Escola Tècnica Superior d'Enginyeria Agraria de Lleida (España). Dept. de Producció Animal | Álvarez Rodríguez, J., Escola Tècnica Superior d'Enginyeria Agraria de Lleida (España). Dept. de Producció Animal | Babot, D., Escola Tècnica Superior d'Enginyeria Agraria de Lleida (España). Dept. de Producció Animal | Hermida, B., Escola Tècnica Superior d'Enginyeria Agraria de Lleida (España). Dept. de Producció Animal | Yáñez, D.R., Consejo Superior de Investigaciones Científicas, Granada (España). Estación Experimental del Zaidín | Abecia, L., Consejo Superior de Investigaciones Científicas, Granada (España). Estación Experimental del Zaidín | Balcells, J., Escola Tècnica Superior d'Enginyeria Agraria de Lleida (España). Dept. de Producció Animal
This experiment studied the effects of slurry concentration (% DM), co-substrate addition and cow faeces as external source of metanogenic Archaea (MA) on "in vitro" evolution of bacterial and MA titres along the anaerobic fermentation of pig slurry (56 day incubation period). The artificial constructed slurry (10 % DM from fresh faeces and urine) was diluted to 2, 4 and 6% DM using the mineral Theodorou's buffer. Cereal straw, sugar beet or apple pulpwere used as substrates. Methane production was determined by gas cromatography and bacterial titres were determined by quantitative real-time PCR (qPCR). The relative proportion of MA was modified by slurry concentration, being lower in those slurries with high DM content (P<0.05). Addition of cow faeces has no effect on the diluted (2 % DM) although it showed a differential response in the concentrated slurries. MA titles were reduced in 4% DM slurry with cow faeces addition whereas the opposite occurred in 6% DM slurry. No substrate effect was detected on MA titles. A negative correlation was detected between methane production (ml/g OM) and MA titles, suggesting that only specific Archaea are able survive and to produce methane on anaerobic slurry fermentation conditions.
Показать больше [+] Меньше [-]