Distribution of Major Allelic Variants at Exon-IV of Kappa Casein Gene in Indian Native Cattle
2010
Sodhi, M. | Mishra, B.P. | Prakash, B. | Kaushik, R. | Singh, K.P. | Mukesh, M.
Thepresent study was aimed to screen the pattern of major allelic variants of kappa casein (/(-CN) locus across 744 animals representing 17 Indian native cattle breeds adapted to different agroclimatic regions. A 935 bp fragment of /(-CN exon IV region was PCR amplified and digested with HindllI and HaellI restriction enzymes to reveal the frequency distribution of A, Band E alleles. The digestion with HindllI pattern revealed a single 935 bp fragment or three fragments of 935, 520 and 415 bps, whereas HaellI digestion displayed a monomorphic pattern of 641 and 294 bps fragments in all the studied samples. On analysis, only two alleles A and B were detected. AA genotype was most commonly distributed (0.818), with predominance of A allele (0.908). The present analysis indicated contrasting allelic pattern at /(-CN locus in Indian native cattle in comparison to that reported for Bos taurus.
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Эту запись предоставил Indian Council of Agricultural Research