Isolation and invitro study of the pathogenesity of a Fusarium oxysporum Schlecht. isolate on larvae of Sart longhorn beetle, Aeolesthes sarta Solsky (Col. Cerambycidae)
2009
Farashiani, M. E., Research Institute of Forests and Rangelands, Tehran, Iran | Moradali, M. F., Research Institute of Forests and Rangelands, Tehran, Iran | Zamani, S. M., Research Institute of Forests and Rangelands, Tehran, Iran | Askary, H., Research Institute of Forests and Rangelands, Tehran, Iran | Ajamhasani, M., Research Institute of Forests and Rangelands, Tehran, Iran | Hoseinkhani, H., Research Institute of Forests and Rangelands, Tehran, Iran | Zahedi, M., Research Institute of Forests and Rangelands, Tehran, Iran | Ghasemi, R., Research Institute of Forests and Rangelands, Tehran, Iran | Ehteshamhoseini, M., Research Institute of Forests and Rangelands, Tehran, Iran | Zeinali, S., Research Institute of Forests and Rangelands, Tehran, Iran
Fusarium oxysporum is generally known as a saprophytic and plant pathogenic agent. There are several reports that indicated this fungus as a entomopathogenic agent on several insect species. In this study a new isolate of this fungus is recorded for the first time on larvae of A. sarta in Tehran and named Sarta. Infections due to F. oxysporum have been frequently observed on larvae of A. sarta in some regions of Iran. Furthermore, in this study, pathogenicity of this strain is subjected to bioassay larvae of A. sarta in laboratory condition. In this regard a completely randomized block design with 3 replications and 5 treatments was used. Experiment were conducted to determine efficacy of various treatments (1 ×105, 1 ×106, 1 ×107, 1 ×108 spore/ml) and control (distilled water) against larvae of A. sarta. The larvae were similar in size and age and they were individually treated with aqueous concentrations for 0.5 minute. After inoculation the larvae were placed in artificially made galleries in the sapwood of poplar trunks. After 15 days, the trunks were chopped and mortality rate of larvae was determined. Data were analyzed by Probit program and LC50 and LC90 concentrations were estimated. The results indicated that all of the treatments significantly different from the control (P0.01). Mortality rate of various treatments (1 ×105, 1 ×106, 1 ×107, 1 ×108 spore/ml) and control were 39.44, 43.61, 60, 64.17 and 0%, respectively. This suggest that by increasing the concentration, mortality rate increases and LC50 and LC90 level were 3.02 × 106 and 5.04 × 1010 spore/ml., respectively. The entomopathogen can propound in biological control of the pest and supplementary studies must be continued.
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