Effect of different levels of molasses, sulfur and urea on in vitro digestibility and fermentibility of buffaloes rumen liqure

This experiment was down with the goal of preliminary increase buffalo rumen situation and enhanced the potential of digestibility and fermentibility of rouphages in laboratory condition. For investigation the effect of differen1levels of molasses, sui fur and urea on fermentibility and digestibility of bnffalo diets, were used a compeletely randomized design in factorial arrangment. The first factor were molasses, sui fur and later factor were urea. For cause that, with increase levels of molasses, increased sulfur of diets, molasses and sulfur together, were assumed a facctor with 4 levels (5, 10, 15 and 20 percent molasses, and 0.33, 0.37, 0.41 and 0.45 percent sulfur, per dry matter) and later factor urea with 3 level(0.5, 1 and 1.5 percent urea, per dry matter) and with 5 replicates. 12 diets were prepared in forage and concentrate sigments with regards of requirments of dairy buffaloes with 600 kg body weight and 10 kg daily milk yield with 4% FCM(NRC 1989). Rumen fluid was obtained from 4 ruminally fistulated male buffaloes. In vitro dry matter and organic matter digestibility were measured according to method of two step digestion rumen liqure and pepsin (Tilley & Terry 1963) and for investigation of rumen fermentibility and gas production, were used the method of Ivlenke et al 1979. Different levels of molasses, sui fur and urea, were not significant affected on dry matter and organic matter digestibility (P0.05).lTom ] 2h incubation time toward later incubation hours, were observed significant differences between means of gas production for addition of different levels of molasses, sulfur and urea for diets(P0.05). For reason negative correlation between ep content and gas produntion of diets, data of cummulative gas production, corrected for percent of urea in different diets. Investigation of simple and intraction effects of different levels of molasses, sulfur and urea, on cummulative, noncummulative and corrected cummulative gas production, indicated significant decrease on rumen fermentibility at lavels of molasses 20%, suI fur 0.45% (M4S4) and urea 1.5% (D3) (P0.05). Results of this experiment, indicated that, probably use of 15% molasses with 0.41 % sulfur and 1% urea per dry matter of diets were not negative effects on digestibility and fermentibility of roughage matterial in diets with 60% forages.