Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)
2009
Gao, Feng-Shan, Dalian University, Dalian, Liaoning, China | Hu, Gui-Xue, Dalian University, Dalian, Liaoning, China | Xia, Xian-zhu, Institute of Military veterinary Science, Academy of military medical Science, Changchun, Jilin, China | Gao, Yu-Wei, Institute of Military veterinary Science, Academy of military medical Science, Changchun, Jilin, China | Bai, Ya-Duo, College of Animal Science of Jilin Agricultural University, Changchun, Jilin, China | Zou, Xiao-Huan, Institute of Military veterinary Science, Academy of military medical Science, Changchun, Jilin, China
Two giant pandas (Ailuropoda melanoleuca) died of unknown causes in a Chinese zoo. The clinical disease profile suggested that the pandas may have suffered a viral infection. Therefore, a series of detection including virus isolation, electron microscopy, cytobiological assay, serum neutralization and RT-PCR were used to identify the virus. It was determined that the isolated virus was a canine coronavirus (CCV), on the basis of coronavirus, neutralization by canine anti-CCV serum, and 84.3% to 100% amino acid sequence similarity with CCV. The results suggest that the affected pandas had been infected with CCV.
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