Root and butt rot causing fungi: the impact of broadleaved species admixture on the incidence of Heterobasidion spp. in spruce stands | Sakņu un stumbra trupi izraisošo sēņu sugu sastopamība egļu audzēs – lapu koku piemistrojuma ietekme uz Heterobasidion spp. izplatību
2010
Arhipova, N., Latvian State Forest Research Inst. Silava, Salaspils (Latvia) | Donis, J., Latvian State Forest Research Inst. Silava, Salaspils (Latvia) | Gaitnieks, T., Latvian State Forest Research Inst. Silava, Salaspils (Latvia) | Liepa, I., Latvia Univ. of Agriculture, Jelgava (Latvia). Forest Faculty. Dept. of Silviculture
During 2005-2006, incidence of root and butt rot in spruce stands and the presence of fungi responsible for it were investigated. Surveyed were altogether 319 stands dominated by Norway spruce (Picea abies) (162 clear-felled and 157 thinned stands) in the Vidusdaugava, Ziemelkurzeme, Zemgale, and Ziemellatgale Forest Districts. In total, more than 25,000 spruce stumps were measured (15-240 stumps per stand) and the number of decayed stumps was recorded, using the transect method. To identify decay-causing fungi, wood samples from 1,182 decayed stumps were taken (1-12 samples per stand). In addition, 114 decayed spruces were cut down in four spruce stands in the Zemgale and Vidusdaugava Forest Districts and wood samples also were taken. From all samples fungal isolations were made and fungal species identified, using morphological and molecular techniques. PCR (polymerases chain reaction) were made using the primers ITS 1F and ITS 4. Sequencing was made by the Korean company Macrogen and sequences were manually edited, using the Seqmen software. For fungal species identification the NCBI and the sequence databases of the Swedish University of Agricultural Sciences were used. Heterobasidion species were identified, using the intersterility test. Root rot incidence in analyzed spruce stands was 22.6 - 13.8%. Heterobasidion parviporum, isolated from 11.1% of spruce stumps and 55.2% of freshly cut trees, turned out to be the most widespread fungus causing root and butt rot, followed by Stereum sanguinolentum, which was found in 3% of spruce stumps and 3.5% of freshly cut trees. Studied was also the impact of broadleaved species admixture on the incidence of root and butt rot.
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