Reducing/antioxidant activity of fungal and algal samples in a ferric-tripyridyltriazine reduction microassay
2002
Lou, Phing How, Institute of Postgraduate Studies and Research | Cheng, Hwee Ming, Malaya Univ. (UM), Kuala Lumpur (Malaysia). Faculty of Medicie. Dept. of Physiology | Chang, Yu Shyun, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Kuala Lumpur (Malaysia) | Chu, Wan Loy, International Medical University, Kuala Lumpur (Malaysia) | Sam, Choon Kok (eds.), Institute of Postgraduate Studies and Research
The ferric reducing/ antioxidant power (FRAP) assay is a simple test which depends on the reduction of ferric-tripyridyltriazine (FellLTPTZ) complex to the ferrous form (Fell), resulting in an intense blue chromogen with maximum absorption at 593 TIm. Based on the corresponding reactions of a Fell standard solution tested in parallel, the FRAP reaction produced can be linearly related to the molar concentration of the potential reducing properties present within solutions, containing an antioxidant in purified form and also whole preparations of biological samples (algae, fungi, plant materials). Using ascorbic acid as a standard, the relative activity of four algal and five fungal samples were measured and expressed as FRAP values (µmol/l of equivalent ascorbic acid activity in FRAP assay). The FRAP values obtained for the former were 288 for Spirulina platensis, 97 for Chlorococcum sp 102, 32 for Chlorococcum sp 074, and 31 for Pavlova luthe ri. For fungal samples at the same concentration of 10 mg/ml in methanol, Penicillium, Aspergillus and an unidentified species exhibited strong FRAP reactions (1000, 392, and 549 respectively). The potential of the FRAP assay lies in its features: inexpensive reagents, straightforward procedure, short reaction time and highly reproducible results over a wide concentration range. The adaptation of the FRAP assay to the microplate format has made this method an even more rapid test and it allows simultaneous assessment of many samples. These factors combined make the FRAP assay a useful complementary technique for preliminary antioxidant assessment of biological samples or fluids.
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