Evaluation of sialic acid and acute phase proteins (haptoglobin and serum amyloid A) in clinical and subclinical bovine mastitis
2011
Nazifi, S. (Shiraz Univ. (Iran). Dept. of Clinical Studies) | Haghkhah, M. (Shiraz Univ. (Iran). Dept. of Pathobiology) | Asadi, Z. (Shiraz Univ. (Iran). Dept. of Clinical Studies) | Ansari-Lari, M. (Shiraz Univ. (Iran). Dept. of Food Hygiene) | Tabandeh, M.R. (Shahid Chamran Univ. of Ahvaz (Iran). Dept. of Biochemistry) | Esmailnezhad, Z. | Agamiri, M.
The present study was conducted to evaluate the concentrations of sialic acids (total, lipid bound and protein bound) and their correlation with acute phase proteins (haptoglobin and serum amyloid A) in clinical and sub-clinical mastitis of cattle. Thirty sub-clinical mastitic cows with positive California mastitis test (CMT) test and no clinical signs of mastitis, 10 clinical mastitic cows and 10 healthy cows with negative CMT test and normal somatic cell count were selected. Milk and blood samples were collected after confirmation of clinical and sub-clinical mastitis by somatic cell count and bacterial identification. Serum haptoglobin (Hp), serum amyloid A (SAA), total sialic acid (TSA), lipid bound sialic acid (LBSA) and protein bound sialic acid (PBSA) were measured by validated standard methods. Haptoglobin and SAA increased significantly in both types of mastitis compared with control group (P less than 0.001). However, the ratio of HP/SAA was significantly different
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Эту запись предоставил National Agricultural Research Centre