Impact of dibutyl phthalate and benzyl butyl phthalate on motility parameters of sperm from the European pikeperch Sander lucioperca (L.)
2010
Jarmolowicz, S. | Demska-Zakes, K. | Kowalski, R. | Cejko, B.I. | Glogowski, J.,Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research | Zakes, Z.,Inland Fisheries Institute, Olsztyn (Poland). Dept. of Aquaculture
The impact of dibutyl phthalate (DBP) and benzyl butyl phthalate (BBP) at concentrations of 100, 1000, and 10000 ľg DBP/dm3 and 20, 200, and 2000 ľg BBP/dm3 on the motility parameters of the sperm of European pikeperch, Sander lucioperca (L.), using computer assisted sperm analysis (CASA) was evaluated. The in vitro studies were conducted in the seminal plasma, which is the natural environment of the sperm, using two methods. The first was with the immediate activation of sperm movement in the presence of phthalates (0 h), while the second utilized phthalate incubation for 4, 24, and 48 h. It was confirmed that none of the compounds analyzed had a significant impact on the percentage of motile sperm (MOT), average path velocity (VAP), curvilinear velocity (VCL), straight line velocity (VSL), or amplitude of lateral sperm head displacement (ALH)
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