Phenotypic and molecular variability of boron tolerance in wheat. [Doctoral dissertation] | Fenotipska i molekularna varijabilnost tolerantnosti pšenice na suvišak bora. Doktorska disertacija
2009
Brdar-Jokanović, M,
Excessive amounts of soil boron may cause substantial yield losses in wheat. The most promising approach to solve problems associated with boron toxicity is the identification of boron tolerant wheat genotypes that can be exploited for the development of high-yielding tolerant cultivars. The variation among sixty divergent bread wheat (Triticum aestivum L.) genotypes in response to high boron concentrations has been analyzed under both laboratory and field conditions. The possibility of using molecular markers-microsatellites for identification of boron tolerant wheat genotypes has been also examined. Root length reduction on boron treatments with respect to control was used as selection criterion for boron tolerance under laboratory conditions. In field condition, the selection criterion for boron tolerance was yield reduction on boron treatments with respect to control. Significant phenotypic differences in terms of boron tolerance have been found among analyzed wheat genotypes under both laboratory and field conditions. The allelic polymorphism of examined microsatellite loci was revealed and the indication of the association marker - boron tolerance was found. The performed laboratory method for screening boron tolerance provides fairly good estimate of field boron tolerance. The results of laboratory and field trials, as well as the fact that for our agricultural environment are required the cultivars adapted on wide range of soil boron concentration, imply the genotypes NS 73/02, Ljiljana and Mina as good starting material for breeding boron tolerant cultivars of wheat.
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