Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle disease virus envelope in vitro | Ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti in vitro
2006
Nišavić, J., Fakultet veterinarske medicine, Beograd (Serbia) | Milić, N., Fakultet veterinarske medicine, Beograd (Serbia)
The objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum.
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