[Application of REP-PCR and some classical methods for detection of Xanthomonas arboricola pv. pruni] | Primena Rep-PCR i nekih klasičnih metoda u detekciji Xanthomonas arboricola pv. pruni
2009
Gašić, K., Poljoprivredni fakultet, Beograd - Zemun (Serbia) | Obradović, A., Poljoprivredni fakultet, Beograd - Zemun (Serbia)
Xanthomonas arboricola pv. pruni represents economically very important pathogen of stone fruits worldwide. This bacterium has not been detected in Serbia, and therefore is considered quarantine pathogen in this country. Its status in European countries and in Serbia requires inspection of imported planting material at the border check points, in order to protect introduction of the pathogen in our country. Therefore, methods for detection and identification of this pathogen were proposed and established by European Plant Protection Organization (EPPO). Absence of the bacterium in Serbia consequently resulted in lack of practical experience in symptomatology of the disease as well as detection methods. Thus, in collaboration with the colleagues from University of Modena and Reggio Emilia, Italy, we acquired common laboratory techniques recommended by EPPO for preliminary diagnosis and detection of the pathogen and enabled their routine use in Laboratory for phytobacteriology, Faculty of Agriculture, Belgrade. In this paper we presented our practical experience in applying some of these methods.
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