Ligninolytic enzyme production by Lenzites betulinus on selected plant raw materials
Knežević, A.Z., Faculty of Biology, Belgrade (Serbia) | Milovanović, I.N., Faculty of Biology, Belgrade (Serbia) | Stajić, M.M., Faculty of Biology, Belgrade (Serbia) | Vukojević, J.B., Faculty of Biology, Belgrade (Serbia)
To get a better insight into the ligninolytic system of Lenzites betulinus, the effect of wheat straw and oak sawdust, as carbon sources, on production of Mn-oxidizing peroxidases and laccase, under solid-state and submerged fermentation, was studied. Obtained results revealed considerable differences related to the both factors affecting enzyme activities. Wheat straw was more favorable carbon source for Mn-oxidizing peroxidases and oak sawdust for laccase activity. Solid-state fermentation of wheat straw was optimal for Mn-dependent peroxidase activity (72.1 U/l). In contrary to this, submerged fermentation of the same residue gave the highest level of versatile peroxidase activity (25.4 U/l). The peak of laccase activity was noted during solid-state fermentation of oak sawdust (32.3 U/l), while this enzyme was not detected under submerged fermentation of any plant residues.
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