Determination of trans fatty acids in foodstuffs by gas chromatography - mass spectrometry. [Doctoral dissertation] | Određivanje trans masnih kiselina u prehrambenim proizvodima gasnom hromatografijom - masenom spektrometrijom. Doktorska disertacija
2010
Kravić, S.Ž.
In this thesis, operating conditions for fatty acids determination, including trans isomers, by capillary gas chromatography-mass spectrometry, on capillary column SP-2560 were optimized. Temperature program, split ratio and condition of data acquisition by SCAN technique were optimized using standard solutions of fatty acids methyl eters. A sample preparation method based on simultaneous microwave assisted extraction-esterification (SMEE) was developed for the determination of the fatty acid composition of foodstuffs by gas chromatography-mass spectrometry. The results showed that compared to the conventional method, SMEE method offer the advantages of short sample preparation time, low consumption of expensive organic solvents and less energy consumption. This good agreement between results provided, both by the SMEE and reference method, demonstrates the usefulness of the former as the routine method for the treatment of food samples prior to trans fatty acid analysis. The fatty acid composition , and trans fatty acid content of 273 samples collected from June 2006 to June 2009 year were determined. Trans fatty acid content in the analyzed samples of food products, raw materials and intermediate products used in bakery and confectionary industry was ranged in an a very wide interval, from 0.0% to 48.7%.
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