The expression profiles of selected genes in leaves of different beans species (Phaseolus spp.) under drought stress
2008
Meglič, V., Agricultural Institute of Slovenia, Ljubljana (Slovenia) | Kavar, T., Agricultural Institute of Slovenia, Ljubljana (Slovenia) | Maras, M., Agricultural Institute of Slovenia, Ljubljana (Slovenia) | Kidrič, M., Jožef Stefan Institute, Ljubljana (Slovenia) | Šuštar-Vozlič, J., Agricultural Institute of Slovenia, Ljubljana (Slovenia)
In order to compare the response to drought stress in leaves of Phaseolus coccineus, P. lunatus and P. acutifolius with previously studied P. vulgaris, relative gene expression analysis using qPCR have been carried out for one putative DREB gene and 15 dehydration-responsive transcripts, which had been confirmed as up- or down-regulated in leaves of P. vulgaris previously. Ten transcripts and the reference gene (actin) were successfully amplified in all three species by primers previously developed for P. vulgaris, one transcript (DD5) was amplified only in P.coccineus and P.acutifolius, and five transcripts (CG10,CA1,CG20,25CA145 and DD8) did not amplify in any species. Relevant homologs transcripts CA1 and 25CA145 were amplified using novel primers, designed according to P.coccineus sequences. For these 13 transcripts, expression profiles of Phaseoulus spp. analysed were congruent with the P. vulgaris profiles.
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