Morphological and molecular identification of Fusarium spp. isolates originating from tomato | Morfološka i molekularna identifikacija izolata Fusarium spp. poreklom sa paradajza
2011
Ignjatov, M., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Petrović, D., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Nikolić, Z., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Jovičić, D., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)
Pathogenic fungi of the genus Fusarium, the causal agents of plant wilt, also occur as parasites of vegetables, causing root and basal stem deterioration and plant wilt. Tomatoes are parasitized by a host of pathogens, including Fusarium oxysporum f. sp. lycopersici, the causal organism of Fusarium wilt. The objective of this paper was to isolate, determinate and identify the causal organisms of tomato wilt based on the morphological and molecular characteristics of pathogen. In July and August of 2009, samples of diseased plants showing the symptoms of tomato wilt were collected for the purposes of identification. The isolation and morphological determination of the fungus were performed on the PDA and CLA nutrient media. In determining the fungal isolates, the polymerase chain reaction (PCR) was also used. The EF1/EF2 primer pair was used for the molecular identification of F. oxysporum isolates. The analyzed samples were found to contain DNA fragments 700 bp in size, which are characteristic of the fungus Fusarium oxysporum.
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