Rapid in vitro propagation of Ocimum sanctum L. through multiple shoot induction
2010
Ramanuj, R.K. | Ramavat, J.M. | Bapodariya, Y.M. | Chariya, L.D. | Mandaliya, V.B. | Thaker, V.S., Saurashtra University, Rajkot (India). Department of Biosciences, Centre for Advanced Studies in Plant Biotechnology and Genetic Engineering
Ocimum sanctum L., known for its great medicinal values, is used in this experiment. In vitro propagation was done for multiple shoot regeneration. To reduce the cost, time and attempts, endogenous concentration of phytohormones are estimated using immunoassay. The new optimised protocol was devised for rapid in vitro propagation of plant through multiple shoot induction. The results achieved in the MS medium where, phytohormones 3.0-5.0 mg/l BAP and 0-0.10 mg/l NAA, and 4.0 mg/l adenine sulphate were tested. The highest number of multiple shoot (23.13 shoots/explant) was obtained along with 2.7 cm shoot length. The phytohormone combination of 3.0 mg/l BAP and 0.05 mg/l NAA was optimum.
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