Formation of a structure of bacterial cenosis in water tips of sugar beet root plants | Формирование структуры бактериального сообщества в отвалах заводов по переработке сахарной свеклы
2010
Akhtemova, G.A. | Pershina, E.V. | Pinaev, A.G. | Andronov, E.E. | Shtark, O.Yu. | Chebotar', V.K. | Borisov, A.Yu. | Tikhonovich, I.A., All-Russia Research and Development Inst. of Agricultural Microbiology, St. Petersburg (Russian Federation) | Kiprushkina, E.I ., St. Petersburg State Univ. of Low-Temperature and Food Processes (Russian Federation) | Abdurashitov, S.F., Ukrainian Academy of Agrarian Sciences, Chernigov (Ukraine). Inst. of Agricultural Microbiology | Dzhianinazzi-Pirson, V., National Inst. of Agronomic Researches, Paris (France)
At sugar mills great volumes of filtration washing sludges (FWS) are accumulated which is a mixture of a filtration sludge and soil and a rich substrate for developing different groups of microorganisms, an analysis of the structure of bacterial FWS community is required to create methodology for reutilization of waste, introducing it in the process of soil formation and recultivation of wasteland. FWS samples were collected at filtration fields where 1-2-year waste was stored and territory where the age of dumps reached 3, 20 and 30 years. The samples of the first storage year FWS were characterized by a pronounced alkaline reaction, the second year storage samples has s low alkaline reaction, the 20-30-year old dumps has a low alkaline or neutral reaction of the substrate. The content of arsenic, cadmium, mercury was below the MAC, the content of lead exceeded the allowable norms 10 times, zinc – almost 5 times. By the third storage year the number of morphotypes increased to 15, and a total number of bacteria twice reduced. 20-30 year old FWS had noticeably reduced total number of bacteria. From the FWS samples of different age a total of 67 morphotypes of cultivated bacteria was isolated, 5 of them were defined as dominant species. In the first storage year dumps dominating are gram-positive non-spore-forming bacteria of g. Microbacterium and spore-forming bacteria of g. Bacillus, rhizosphere microflora starts developing, rare vegetation appears. In many-year dumps an interchange of dominant microflora took place, predominating bacteria were G. Arthrobacter, subdominant were bacteria G. Pseudomonas. A Clorella test showed the absence of phytotoxicity of the studied strains. Testing the ability of synthesizing vitamins positive results were given by strains B. megaterium and A. sulfonivorans. Strain 02B of B.megaterium can be recommended to create industrial microbial preparations for ecologically-oriented agriculture
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