Studies on the phenolic compounds in berries of vitis vinefera cv. thompson seedless lines | 欧洲葡萄品种‘无核白’不同营养系果实的酚类物质

Китайский. 目的更有效地发挥欧洲葡萄品种‘无核白’不同营养系鲜食或制干的用途，筛选‘无核白’系列大粒与白藜芦醇含量较高的鲜食品系，酚类物质浓度与多酚氧化酶活性较低而适宜绿色葡萄干制备的制干品系。方法以‘无核白’葡萄为对照，以其性状上有变异的10个营养系成熟果实为材料，取样测定其果粒重，用折光仪测定可溶性固形物含量，HPLC法测定果皮与全果白藜芦醇含量、酚类物质，比色法测定多酚氧化酶活性。结果大粒无核白和W3等营养系浆果单粒重为对照的1.3286―1.4682倍；W2、W8、长穗无核白和W7等营养系果实白藜芦醇含量为对照的110.65%―128.41%；‘无核白’葡萄营养系果实中总酚含量具有明显差异，含量较低的有长粒无核白、W8、W3和大粒无核白等；不同营养系果皮中多酚氧化酶活性是全果的1.06―13.19倍。结论与对照相比，供试10个‘无核白’葡萄营养系中，单粒重、可溶性固形物含量、果皮与全果的白藜芦醇含量较高的有W3、大粒无核白、长粒无核白和W5等；酚类物质总量较低的营养系有大粒无核白、长粒无核白、W3等；多酚氧化酶活性较低的营养系有W7、长穗无核白、W3、W8。本研究结果为‘无核白’系列无核葡萄品种选育提供了部分依据。

Английский. Objective This research was aimed to evaluate the clonal lines of V. vinefera cv. Thompson Seedless for breeding of new table grape varieties of higher resveratrol concentration, or new raisin grape varieties of lower total phenolic compound (TPC) concentration and polyphenol oxidase (PPO) activity relatively. Method Using V. vinefera cv. Thompson Seedless and ten diverse lines of this variety as materials, fresh berry mass and soluble solid content (TSS), resveratrol，TPCs and PPO activity were evaluated. Result The results indicated that the berry mass of Big-berry Thompson Seedless and W3 were 1.3286-1.4682 times of that of the control (Thompson Seedless). Resveratrol contents of W2, W8, Long-bunch Thompson Seedless and W7 were 110.65%-128.41% of that of the control. TPC contents of each lines were significantly different, in which Long-berry Thompson Seedless, W8, W3, and Big-berry Thompson Seedless were with small amount TPCs. PPO activity of berry skin was 1.06-13.19 times of that of the whole berry.ConclusionCompared to the control, W3, Big-berry Thompson Seedless, Long-berry Thompson Seedless and W5 were superior for fresh table grape breeding based on berry mass, TSS, resveratrol content. TPCs of Big-berry Thompson Seedless, Long-berry Thompson Seedless and W3 were relatively lower than other lines, and PPO activity of W7, Long-bunch Thompson Seedless, W3, W8 were relatively low among the materials tested. These results could provide some indications for the prospective breeding of new lines of Thompson Seedless variety.