Effects of exogenous pea-ferritin gene on the important biological features of rice using near isogenic lines | 利用近等基因系研究豌豆铁蛋白基因对水稻重要生物学特性的影响

Китайский. 为研究外源豌豆铁蛋白基因(Pea-Fer)导入水稻后对受体重要生物学特性的影响，本实验以原始受体亲本粳稻(Oryza sativa ssp.japanica)品种秀水11为轮回亲本，外源豌豆(Pisum sativum)铁蛋白转基因纯系Fer34为非轮回亲本，采取连续回交，结合gus标记基因辅助选择技术，在BC6F3获得含Pea-Fer的Fer34-XS11，它与秀水11构成一对近等基因系。结果显示：(1) Pea-Fer导入显著提高了水稻籽粒中的铁含量，但对镁、钙、锰和锌等的积累作用影响不大；(2) Pea-Fer导入对种子发芽特性和苗期植株生长速率无显著影响；(3) Pea-Fer导入没有改变成株叶片光合色素的含量和组成，但一定程度上提高了植株的净光合速率；(4) Pea-Fer 导入对水稻的成熟期农艺性状及经济性状均无不良影响。外源豌豆铁蛋白基因的导入除使水稻籽粒的铁含量有明显增加外，研究结果表明对水稻其它重要生物学性状并无明显负作用，这为开展富铁水稻新品种培育提供了依据。

Английский. In the present study, we investigated the effects of exogenous pea ferritin gene (Pea-Fer) on the important biological properties of transgenic rice. Fer34-XS11 was obtained from a near isogenic line (NIL BC6F3) of donor parent (exogenous ferritin transgenic pure lines Fer34) and recurrent parent (Oryza sativa ssp.japanica) (Xiushui 11) by successive backcrossing and GUS analysis. Several important biological properties were investigated completely using Fer34-XS11 and Xiushui 11. The major results are as follows: 1)The iron content of Fer34-XS11 was increased significantly, but no obvious difference was observed in the other mineral elements, such as Mg, Ca, Mn and Zn. 2)The introduction of exogenous gene (Pea-Fer) had no significant effect on germination rate and seedling leafing rate. 3) No significant changes were found in the contents and composition of chlorophyll in leaves, but the net photosynthetic rate was dramatically enhanced in the transgenic rice. And 4) no harmful effect were found on major agronomic traits as well as economic traits. All the results showed that the exogenous gene (Pea-Fer) was highly expressed in transgenic rice with increasing the iron content of rice grains. And transferring the exogenous gene (Pea-Fer) did not result in significant harmful effect on the important biological properties. The results provide some theory basis for breeding novel high-iron rice varieties by utilizing this foreign gene.