Potential of chlorophyll fluorescence and VIS/NIR spectroscopy measurement use for the detection of nitrogen content and disease infection of apple leaves
2011
Spacilova, V., Agrotest Fyto, Kromeriz (Czech Republic) | Safrankova, I., Mendelova Univ., Brno (Czech Republic). Ustav Pestovani, Slechteni Rostlin a Rostlinolekarstvi
A possibility of using spectral methods for determining a nutritional status and detecting pathogens in apple trees cvs. 'Jonagold' and 'Idared' was verified in an orchard and pot experiments in 2007-2010. Treatments differed in the fertilizer or fungicide dose. Leaf samples were collected from the experimental variants to determine nitrogen content and to measure spectral reflectance and chlorophyll fluorescence imaging. The occurrence of fungal pathogens Venturia inaequalis and Podosphaera leucotricha was assessed and changes of photochemical efficiency of PSII of infected leaves were evaluated. When verifying the relationship between N concentration in apple-tree leaves and measured chlorophyll fluorescence parameters, a significant relationship was confirmed for the effective quantum yield of PSII and a relationship for the parameters Fv/Fm. A significant difference between healthy leaves and leaves infected by the pathogens studied was confirmed using the spectrophotometer.
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