Constitutive expression of Arabidopsis DREB1B in transgenic potato enhances drought and freezing tolerance
Movahedi, S., Isfahan Univ. of Technology (Iran Islamic Republic). Dept. of Agricultural Biotechnology | Sayed Tabatabaei, B.E., Isfahan Univ. of Technology (Iran Islamic Republic). Dept. of Agricultural Biotechnology | Alizade, H., University of Tehran (Iran Islamic Republic). Dept. of Agricultural Biotechnology | Ghobadi, C., Isfahan Univ. of Technology (Iran Islamic Republic). Dept. of Agricultural Biotechnology | Yamchi, A., Gorgan Univ. of Agricultural Sciences and Natural Resources (Iran Islamic Republic). Dept. of Plant Breeding and Biotechnology | Khaksar, G., Isfahan Univ. of Technology (Iran Islamic Republic). Dept. of Agricultural Biotechnology
DNA cassette consisting of an Arabidopsis dehydration-responsive element binding factor 1 (DREB1B) cDNA, driven by a cauliflower mosaic virus 35S promoter, was introduced into potato plants through Agrobacterium tumefaciens-mediated gene transfer. The presence and expression of the gene in transgenic plants were confirmed by the PCR and RT-PCR techniques, respectively. Northern hybridization using a DREB1B cDNA probe revealed high levels of DREB1B expression among the most transgenic lines. Overexpression of DREB1B imparted a significant freezing and drought tolerance gain in the transgenic potato lines. In comparison with the wild-type plants, the transgenic potatoes contained higher proline content under drought and freezing conditions, and maintained their relative water content higher under water stress. The enhancement of tolerance in transgenic potato highlights the presence of genes responding to the transcription factor DREB1B in this plant.
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