Sequence analysis of the mtDNA region correlated with Shaan 2A cytoplasmic male sterility in rapeseed (Brassica napus L.)
Havlickova, L., Jihoceska Univ., Ceske Budejovice (Czech Republic). Biotechnologicke Centrum | Curn, V., Jihoceska Univ., Ceske Budejovice (Czech Republic). Biotechnologicke Centrum | Jozova, E., Jihoceska Univ., Ceske Budejovice (Czech Republic). Biotechnologicke Centrum | Kucera, V., Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Vyvadilova, M., Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic) | Klima, M., Vyzkumny Ustav Rostlinne Vyroby, Prague (Czech Republic)
The aim of Czech breeders is to produce new, improved cultivars of rapeseed based on the Shaan 2A cytoplasmic male sterility (CMS), a widely used CMS system in China. Sterile Shaan 2A CMS line (S; rf/rf), its corresponding maintainers (N; rf/rf) and fertility restorers (S; Rf/Rf) were analyzed on molecular level for the presence of functional CMS cytoplasm. Two new primer pairs covering CMS-associated gene (so called orf224-1) present in Shaan 2A CMS line were developed and selection capability of the developed primers was successfully evaluated. These primers can be used for early selection of plants with functional Shaan 2A CMS system in breeding programmes.
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