Conservation and micropropagation of some Egyptian native caper, capparis spp, as a wild medicinal plant
2011
Elmoriegy, R.A. | Soliman, S.S. | Shalaby, A.F.
The present study aimed to increase the seed germination percentage and to assess the protocol of micropropagation of Egyptian wild Capparris spp (Capparis spinosa and Capparris cartilagenea L, as well as to determine,e the genetic response for germination and micropropagation of them. Seed germination of Capparis spp is known as a complex trait i.e., no germination of caper seed was obtained without scarification. Three different media were used for seed germination i.e., MS media contain 1.0 mg/13 Benzyl Amino Purine (BAP) + 0.1 mg/I Indole-3-acetic acid (IAA) + 0.1 mg/l Gibberellic acid (GA. ), MS + 0.5 mg/I BAP + 0.1 mg/I IAA + 0.1 mg/I GA. and MS + 1.0 mg/I Kinetin (K) + 0.05 mg/I Indole-3 butyric acid (IBA) + 0.1 GA. , the first medium is considered as favorable than the other two. These results proved that, BAP was more suitable for increasing seed germination. In the second experiment using (GA. ) only without scarification showed no increasing in seed germination. Capparis cartilaginea, showed higher response than Capparis spinosa L, for seed germination. For micropropagation, apical tip from seedling of Capparis cartilaginea, were cultured as explants on two types of media varying in BAP concentration. The medium MS + 2 mg/l BAP + 0.05 mg/1 IBA + 0.05 mg/I GA. gave higher frequencies of number of branches per explants than the other. The hormonal balance played an important role for succession of micropropagation of Capparis spp while BAP proved to be benificial for morphogenesis in Capparis spp . This trial is considered the first trial in Egypt for micropropagation using tissue culture in capparis spp.
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