Sperm DNA damage and embryonic development as related to fertility potential of buffalo bulls
2011
Eid,L.N. | Shamiah, Sh.M. | El-Regalaty, H.A.M. | Keraby, F.E.
Conventional semen analysis are not sufficient to evaluate bull's fertility, however it might be used as a preliminary evaluation for predicting fertility potential. sperm DNA integrity may give a better evaluation of bull fertility potential. In the Current study, six mature buffalo bulls were divided into two groups (based on athreshold of 70% motility; as high or low potential fertility). The first objective of the current study was to evaluate DNA fragmentation (measured as Comet value) in the two groups of bulls (three bulls/group) in relation to its potential fertility. The results showed that low potential fertility bulls had significantly (P0.05) more sperm DNA damage;higher (P0.05) sperm abnormal morphology and lower (P0.05) sperm viability than that of sperm from the high potential fertility bulls. Our second objective was to assess whether a relationship exists between bull's sperm DNA damage and bull in vitro fertility. A significant variations (P0.05) in the developmental competence of the embryos to the morula and blastocyst stages were observed. The results also showed a clear negative correlation between bull's potential fertility and DNA integrity at and the ability of the fertilized ova to sustain its development to the morula (r
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