Evaluation of lactate dehydogenase, alkaline phosphatase and aspartate aminotransferase activity in milk as an indicator of subclinical mastitis in Holstein x Hariana cows.

Gera, Sandeep; Guha, Anirban

Evaluation of lactate dehydogenase, alkaline phosphatase and aspartate aminotransferase activity in milk as an indicator of subclinical mastitis in Holstein x Hariana cows.

2011

Gera, Sandeep | Guha, Anirban

Pertaining to the scenario at hand milk samples from Holstein x Hariana crossbred cows were investigated for alternation in enzyme activity as influenced by sub clinical mastitis (SCM) and their percent sensitivity,specificity,accuracy,positive and negative predictive values, positive and negative likelihood ratios and correlation with (somatic cell count) SCC were calculated. It was found that SCM influence significantly the activity of ALP at pO.OIand LDH at pO.O5in milk. Aspartate amino transferase activity was not influenced by SCM in milk. Moreover, both were found to be correlated with somaticcellcount, significantly.Fromthe likelihoodratios itwas clearthat both alkalinephosphatase (ALP) and LDH can be used for diagnosis of SCM, but, ALP is the safe indicator of SCM in the present study.

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