Genetic structure of a Persian partridge population in Animal Science Research Institute of Iran using microsatellite markers
2011
Boroufeh, K., Graduated in Islamic Azad University, Karaj | Amirinia, C., Members of Scientific Board of Animal Sciences Research Institute of Iran | Emrani, H., Members of Scientific Board of Animal Sciences Research Institute of Iran | Noshari, A.R., Member of Scientific Board of Islamic Azad University, Karaj
The aim of this study was take advantages of usefulness potential microsatellite markers in detect Persian partridge Genetic Structure and population variability for the first time.The chukar partridge population was collected from Semnan & Fars province that keeping in Animal Science Research Institute of Iran (ASRI).The number of 120 blood samples were collected and DNA was isolated using Optimized and Modified Salting-out method. eight microsatellite markers using in this study was Aru 1F114¡ Aru1E97¡ Aru1E102¡ Aru 1E66¡ Aru 1B3¡ Aru 1G4¡ Aru 1M127 & Aru 1J76 .The elements and thermal cycle for each locus was optimized before done PCR reaction. After PCR amplification, microsatellite alleles were identified on 8% denaturing polyacrylamide gels,followed by AgNO3 staining so partridges had been genotyped. Data analyses consist of several parameters of population genetics were done using GENEPOP 3.1.All of locus tested for Hardy-Weinberg equilibrium by chi-square(X2) and likelihood ratio(G2). All loci except Aru1E102 were deviated from Hardy-Weinberg equilibrium in population (P0.05). The entire loci were polymorphic.The most number of observed alleles were in Aru1E97 with 14 alleles and the lowest number of alleles were in Aru 1F114 ¡ Aru 1M127& Aru 1J76 with 4 alleles.The average observed number of alleles, Shannon's Information index & ¬ Polymorphic Information Content(PIC) were 6.3750, 1.4568 and 0.6567 respectively.The average observed heterozygosity was 0.4522 and average expected heterozygosity was obtained 0.7017.Results of Genetic variation in Persian partridge population as comparison with other exotic similar studies,detect suitable variation value in Persian native partridge population.
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