Comparison of different DNA extraction methods for molecular study of Fasciola parasites
2011
Mahami-Oskouei, M., Department of Medical Parasitology, Tarbiat Modares University, Tehran, Iran | Dalimi, A., Department of Medical Biotechnology, Tarbiat Modares University, Tehran, Iran | Forouzandeh-Moghadam, M., Department of Medical Biotechnology, Tarbiat Modares University, Tehran, Iran | Rokni, M.B., Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran
Fasciolosis is an important parasitic disease common among humans and livestock. It is considered as a health problem and causes great economic losses in several regions of the world. Considering the prevalence of fascioliasis, study on molecular aspect of the parasite is so much important. The present study was carried out to achieve a suitable method of DNA extraction from Fasciola for further molecular study.After collection of Fasciola, five different DNA extraction methods, including phenol chloroform, Chelex, CTAB, Ish-Horowicz and kit were evaluated. In this evaluation, the quality and quantity of DNA obtained from each method considering OD, DNA concentration and PCR efficiency were investigated. The results indicated that, the highest concentration of DNA, and efficiency of PCR were obtained by using Ish-Horowicz, and the kit respectively. Chelex, CTAB and phenol chloroform methods in comparison with Ish-Horowicz had lower concentration of DNA and the lower PCR efficiency.The method of Ish-Horowicz showed higher DNA quantity in comparison with other methods. But in term of time consuming, DNA contamination and quality and efficiency of PCR is not so favorite method.
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