Optimization of direct and indirect regeneration in pear
2008
Jafarkhani Kermani, Maryam | Habashi, Aliakbar | Abdolahi, Hamid | vatanpour Azghandi, Ali | Emadpour, Masoumeh | Hosseini, Zahra-sadat
Traditional pear breeding involves hybridization between and within different species. However, due to high levels of heterozygosity, polygenic characters and long juvenile stage, traditional breeding is very time consuming and expensive. Therefore, using modern biotechnology methods such as genetic engineering and mutation has become popular. In this regard effective and repeatable in vitro regeneration protocols are the prerequisites for in vitro breeding. The aim of the present project was to develop an effective direct and indirect regeneration method for three commercially important pear cultivars (Dare Gazy, Harrow Delight and Bartlett). For direct regeneration; culture media (Nitsch) containing different concentrations of TDZ (0, 2.5, 5, 7.5 μM) or BAP (0, 4, 8, 16 μM) in combination with NAA (0, 1 μM) and three leaf explants (apical, middle and basal) were compared. For indirect regeneration; ½ MS media containing different concentrations of TDZ (0, 4, 8, 16 μM) in combination with NAA (0, 1, 2 μM) and two leaf explants (apical and basal) were studied. The results indicated that in both regeneration methods maximum regeneration was observed in the leaf basal explants. In both regeneration methods the effect of genotype was very apparent, the highest regeneration was observed in the Dare Gazy and the lowest percentage of regeneration was observed in the Bartlett. Current investigation presented the best hormonal treatment for direct and indirect regeneration. The highest percentage of direct regeneration was observed in the medium containing 7.5 μM TDZ and 1 μM NAA. The highest percentage of regeneration was observed in the medium containing 4 and 2 μM TDZ.
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