Differential patterns of nestin and glial fibrillary acidic protein expression in mouse hippocampus during postnatal development
Kim, J.S., Chonnam National University, Gwangju, Republic of Korea | Kim, J.H., Chonnam National University, Gwangju, Republic of Korea | Kim, Y.J., Chonnam National University, Gwangju, Republic of Korea | Yang, M.Y., Chonnam National University, Gwangju, Republic of Korea | Jang, H.S., Chonnam National University, Gwangju, Republic of Korea | Kang, S.W., Chonnam National University, Gwangju, Republic of Korea | Kim, J.C., Chonnam National University, Gwangju, Republic of Korea | Kim, S.H., Chonnam National University, Gwangju, Republic of Korea | Shin, T.K., Jeju National University, Jeju, Republic of Korea | Moon, C.J., Chonnam National University, Gwangju, Republic of Korea
Intermediate filaments, including nestin and glial fibrillary acidic protein (GFAP), are important for the brain to accommodate neural activities and changes during development. The present study examined the temporal changes of nestin and GFAP protein levels in the postnatal development of the mouse hippocampus. Mouse hippocampi were sampled on post natal day (PND) 1, 3, 6, 18, and 48. Western blot analysis showed that nestin expression was high at PND 1 and markedly decreased until PND 18. Conversely, GFAP expression was acutely increased in the early phase of postnatal development. Nestin immunoreactivity was localized mainly in tilt processes of ramified cells at PND 1, but expression subsequently decreased. In contrast, GFAP was evident mainly in the marginal cells of the hippocampus at PND 1, but immuno reactivity revealed satellite, radial, or ramified shapes of the cells from PND 6-48. This study demonstrates that the opposing pattern of nestin and GFAP expressions in mouse hippocampus during postnatal development occur it. the early development stage (PND 1-18), suggesting that the opposing change of nestin and GFAP in early postnatal development is important for neural differentiation and positioning in the mouse hippocampus.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Korea Agricultural Science Digital Library