Antioxidant and Antigenotoxic Effects of Sansuyu Fruit (Corni fructus) Extracted with Water at Different Temperatures
2011
Lee, M.H., Kyungnam University, Changwon, Republic of Korea | Kim, J.M., Kyungnam University, Changwon, Republic of Korea | Park, E.J., Kyungnam University, Changwon, Republic of Korea
The objective of this study was to evaluate the antioxidant and antigenotoxic activities of sansuyu fruit (Corni fructus, CF) at temperatures of 25℃, 50℃, and 90℃ using a water extraction method. Total phenolic content (TPC), DPPH radical-scavenging activity (RSA), and superoxide dismutase (SOD)-like activity, and ORAC (Oxygen radical absorbance capacity) values were determined. Also the antigenotoxicity of CF was determined by measuring inhibitory effects of H₂O₂ induced DNA damage in human leukocytes using the comet assay. The TPC in the CF extracts was 4.2, 4.6, and 5.5 g/100 g GAE in 25℃, 50℃, and 90℃, respectively. The DPPH RSA of the CF extracts increased in a dose-dependent manner over the range of 50~1000 ㎍/mL in all temperatures and the SC∧50 of DPPH RSA of the CF extracts were not significantly different at different extraction temperatures. The SC∧50 of SOD-like was the highest in CF extracted at 25℃ (1.1 mg/mL) followed by 90℃ (1.2 mg/mL) and 50℃ (1.3 mg/mL). The ORAC values of the CF extracts were not significantly different in low concentration (10 μM/mL) and was in order of 25℃ (5.7 μM TE) less than 90℃ (6.2 μM TE) less than 50℃ (8.5 μM TE) in high concentration (50 μM/mL). 200 μM H₂O₂ induced DNA damages in human leukocytes were significantly reduced by the pretreatment with the CF extracts. These results suggest that sansuyu fruit (Corni fructus) can be used as a natural source for antioxidant activities and as antigenotoxic agents regardless of the water extraction temperature.
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