Protective effect of ethyl acetate extract of Ishige okamurae against carbon tetrachloride-induced acute liver injury in rats
2011
Kang, S.H., Jeju National University, Jeju, Republic of Korea | Yang, W.J., Jeju National University, Jeju, Republic of Korea | Oh, H.S., Jeju National University, Jeju, Republic of Korea | Bae, Y.J., Jeju National University, Jeju, Republic of Korea | Ahn, M.J., Jeju National University, Jeju, Republic of Korea | Kang, M.C., Jeju Technopark, Jeju, Republic of Korea | Ko, R.K., Jeju Technopark, Jeju, Republic of Korea | Kim, G.O., Jeju Technopark, Jeju, Republic of Korea | Lee, J.H., Samsung Changwon Hospital, School of Medicine, Sungkyunkwan University, Changwon, Republic of Korea | Hyun, J.W., Jeju National University, Jeju, Republic of Korea | Moon, C.J., Chonnam National University, Gwangju, Republic of Korea | Shin, T.K., Jeju National University, Jeju, Republic of Korea
Several compounds and extracts isolated from a brown alga, Ishige (I.) okamurae, exhibit anti-oxidant and anti-inflammatory effects. The present study investigated whether the ethyl acetate (EtOAc) fraction of I. okamurae (EFIO) could ameliorate carbon tetrachloride (CCl₄)-induced hepatotoxicity in rats. Sprague-Dawley rats were intraperitoneally (i.p.) administered with EFIO at 10 or 50 mg/kg per day for 2 consecutive days before CCl₄ injection (3.3 mL/kg, i.p.). Twenty four hours later, the rats were anesthesized with diethyl ether and dissected. Pretreatment with EFIO significantly reduced the increased serum levels of alanine aminotransferase and aspartate aminotransferase in CCl₄-treated rats. Pretreatment with EFIO also significantly inhibited the reduced activities of superoxide dismutase and catalase in the CCl₄-injured liver. Histopathological evaluations showed that hemorrhage, hepatocyte necrosis, inflammatory cell infiltration, and fatty degeneration induced by CCl₄ treatment were ameliorated by the administration of EFIO. Additionally, liver immunohistochemical analyses revealed the marked reduction in ED1-positive monocyte-like macrophages in EFIO-pretreated rats given CCl₄. These results suggest that EFIO ameliorates CCl₄-induced liver injury, possibly through the inhibition of oxidative stress.
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