Cellular alterations during accelerated aging of French bean seeds.
1996
Begnami C.N. | Cortelazzo A.L.
Seeds of Phaseolus vulgaris L. were artificially aged by exposing them to 42 deg C and 100 % relative humidity during one to sixteen days. The percentage of germination decreased from 100 % at 0-4 days of accelerated aging (AA) to 22 % at six days and finally to 0 % after 16 days of AA, while the average germination period increased as the AA period was prolonged. The in situ analyses and dosages of storage material revealed a disorganization and partial loss of the protein content during this period. A large number of very small starch grains suggests that degradation occured of this storage material during AA, in spite of the dosages obtained by the Anthrone method which were not significantly different. The nuclei became pyknotic in 81 % of the cells analyzed after 16 days of AA. The nucleoli disappeared during this period, also confirming the viability loss of cotyledon cells during aging.
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