Nucleic acids in photosystem II reaction center preparations extracted using the detergents cholate and deriphat.
Ganago I.B.
Observations indicating the presence of nucleic acids in photosystem II reaction center preparations are described: a) phenol and chloroform extraction produced a material with an absorption spectrum similar to that of nucleic acids; b) agarose gel electrophoresis followed by staining with ethidium bromide produced a fluorescent band with a mobility close to that of bromophenol blue; c) the absorption of the extract at 260 nm increased on heating, as occurs when small nucleic acid molecules are melted; d) treatment of photosystem II reaction centers with streptomycin sulfate produced a precipitate, and the supernatant completely lost photochemical activity. These results suggest that nucleic acids may be present in the form of nucleoprotein complexes with pigments.
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