Evaluation and application of immunochemical methods for fumonisin B1 in corn.
Trucksess M.W. | Abouzied M.M.
Two immunochemical methods were evaluated and compared with a strong anion exchange (SAX) method for the determination of fumonisin B1 (FB1) in corn. The two methods were the enzyme-linked immunosorbent assay (ELISA) and the monoclonal antibody-affinity column (IAC) method. The ELISA test is a direct competitive microtiter-well assay in which a strip reader, which measures the color of an enzymatic reaction, is combined with a log/logit data transformation program and linear regression calibration to determine FB1 concentration. FB1 in test samples from IAC or SAX isolation is derivatized with o-phthaldialdehyde, and the derivative is separated from other impurities by reversed-phase high-performance liquid chromatography (HPLC) and then quantitated by fluorescence detection. Recoveries of FB1 from corn spiked over the range of 1-4 micrograms/g were 73-106, 79-83, and 64-92% for the ELISA, IAC, and SAX methods, respectively. Results of analysis of the same extract from naturally contaminated corn by the three methods were similar. HPLC-electrospray mass spectrometry was used to positively identify the FB1 isolated with the microtiter wells from an extract of a naturally contaminated corn.
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