Comparison study of a fumonisin enzyme immunoassay and high-performance liquid chromatography.
Dreher R.M. | Usleber E.
Corn samples collected from German retail stores were tested for fumonisin using a commercially available enzyme-linked immunosorbent assay (RIDASCREEN Fumonisin) and HPLC. The recoveries for fumonisin B1 from artificially contaminated corn at levels of 50 and 500 ng/g were 59.7% and 73.2% after the enzyme immunoassay (EIA) analysis of the raw extracts; the corresponding values after SAX extraction and HPLC analysis were 62.3% and 65.6%. The comparison of both methods showed a slight overestimation of the EIA versus HPLC, but the RIDASCREEN Fumonisin can be characterized as a powerful and very acceptable screening method.
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