Effects of protein source and co-culture on bovine embryo development in synthetic oviductal fluid medium.
Rorie R.W. | Lester T.D. | Miller G.F. | Gliedt D.W. | McNew R.W.
Experiment 1 compared the development of 2- to 4-cell bovine embryos cultured in synthetic oviductal fluid with 20% fetal calf serum or 3.2% BSA and in the presence of oviductal cells, cumulus cells, or medium alone. More embryos developed in medium with serum, regardless of culture method (P = 0.063). Oviductal cell co-culture resulted in more embryos developing to at least the morula stage (P less than or equal to 0.066). The number of blastocysts was increased by the use of serum instead of BSA in cumulus cell co-culture medium (P < 0.001). Regardless of culture method, a similar percentage of embryos were excellent or good quality when BSA was used in medium (P greater than or equal to 0.400). Addition of serum to oviductal cell co-culture medium increased the number of excellent or good quality embryos (P = 0.019). Experiment 2 further compared the development of 2-cell or 3- to 4-cell embryos co-cultured with oviductal cell suspensions in serum-supplemented synthetic oviductal fluid or M-199 medium. More 3- to 4-cell than 2-cell embryos developed to at least the morula stage (P < 0.001). More embryos developed to at least the morula stage in synthetic oviductal fluid (P = 0.083). Neither initial embryo cell stage nor medium type influenced the percentage of developing embryos that achieved the blastocyst stage or final morphological quality of embryos (P greater than or equal to 0.535).
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