In vitro and macropropagation of the wildflower Dyssodia pentacheta (D.C.) Robins.
Zimmerman T.W. | Davies F.T. Jr. | Zajicek J.M.
Dyssodia pentacheta, a prostrate-growing perennial Texas wildflower with potential for use in low-maintenance landscapes, was propagated in vitro and by stem cuttings under mist. Optimum rooting for IBA-treated semihardwood terminal stem cuttings (3 to 30 millimole IBA) and in vitro-grown nodal segments (30 to 100 millimole IBA) occurred after 4 weeks under an intermittent mist system. A 300-millimole IBA basal dip was lethal to macro- and microcuttings. In vitro, D. pentacheta produced more shoots per nodal explant on Woody Plant Medium (2 g Gelrite/liter) with 1 to 10 micrometer BA than with combinations of BA and 0.5 micrometer NAA. After shoot proliferation, the shoots were subcultured twice and grown on growth regulator-free medium. When maintaining D. pentacheta in vitro on media devoid of plant growth regulators, 1% sucrose was more effective than 2% for promoting shoot growth and suppressing apparent production of phenolics.
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