Kan chak nam hai gerbera klai phan nai lot thotlong.
1985
Rongrong Visessuwan
Seeds of European strain gerbera were germinated in vitro on Murashige and skoog medium (MS, 1962). Shoot multiplication was made on modified Murashige and Skoog medium (MMS, 1974) with added 10 mg/l kinetin and 0.5 mg/l IAA. The shoots were then treated with some mutagens such as gamma ray and colchicine. Acute irradiation with gamma rays of shoots showed that at doses 1-4 krad, flowers survived but growth was decreased as compared to the control. At dose 1 krad the survival of flowers was 100 percent and the treatments at doses 2, 3 , 4 and 5-10 krad caused 25, 46, 50 and 100 percent death, respectively. When shoots were irradiated with 1 krad of gamma ray for 1-3 times, abnormal characters were found in flowers: stunt shoots, divided leaves at the apex, rough and thick leaves or narrow and thin leaves. Some flowers had red strips on the yellow ray florets. Some flowers were larger than the control but not different in chromosome number. Shoots were soaked in colchicine solution for 24 hours at concentrations of 0.025 and 0.05 percent. All flowers died at 0.05 percent treatment. Some flowers survived in 0.025 percent treatment. From cytological studies, it was found that one plant was tetraploid (4x=100), two flowers were mixoploid and the rests were diploid (2x=50).
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