Influences of age and estrous cycle on the number of embryos recovered from superovulated mice.
1992
Ookutsu S. | Koyanagi F.
From mature mice injected PMSG and hCG for the induction of superovulation, embryos were recovered at 65 to 67 hr after hCG injection. The relations between the number of these embryos and age of female, and between the number of embryos and estrous cycle were studied. The number of embryos was the greatest in the youngest group of 6- to 10-week-old females. In the females over 11 weeks old, the number of embryos decreased significantly with an older age (P < 0.01). In the embryos obtained from the females over 21 weeks old, the percentage of 4- to 8 -cell stage embryos decreased, whereas the percentage of 1- to 3-cell stage embryos and morphologically abnormal embryos increased with an older age. The number of embryos obtained from the females injected PMSG at the metestrous-1 stage of estrous cycle was significantly larger than those of the females injected PMSG at other stages (P < 0.01). However, the percentage of embryos in the various developing stage was not different among the stages of estrous cycle. The results suggest that the number of embryos obtained from the superovulated mouse may be influenced significantly by age and estrous cycle.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Wolters Kluwer