Ultra-low-temperature (-196 C degrees) storage of peach and pear pollens.
1989
Jiang Yusheng | Gao Zhujiu
Pollens of peach (Amygdalus persica L.) varieties Bai Feng and Yu Hua Lou and pear (P. pyrifolia Nakai) varieties Xin Shi Ji and Kang De were dried to 10 - 25% water content before being stored in liquid nitrogen. After being stored at ultra low temperatures (-196 C degrees) for 365 days, the pollens thawed still retained viability. The germinating rate in vitro and seed setting rate of the stored pollens approached those of fresh pollens, indicating that the stored pollens exhibited high tolerance to freezing. This method of long-term preservation would help to breed peach or pear varieties and conserve germplasm resources of these species.
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