The use of isozymes to identify teliospores of Tilletia indica.
Bonde M.R. | Peterson G.L. | Matsumoto T.T.
Single-teliospore cultures of Tilletia indica, causal agent of Karnal bunt of wheat, were examined by horizontal starch gel electrophoresis and staining for 50 enzymes. Thirty-two were present in high concentrations and could be resolved. Of 36 presumed isozyme loci, 15 (42%) were polymorphic (having allelic variation) among cultures. The high number of alleles in common (Rogers' coefficient of similarity = 0.83) among isolates of T. indica facilitated their differentiation from those of Tilletia barclayana, the causal agent of kernel smut of rice. Teliospores of the latter are frequently confused with those of T. indica. The average coefficient of similarity comparing T. indica with T. barclayana was extremely low (0.04), emphasizing the large difference in their isozymes. Isozymes are being used at the Foreign Disease-Weed Science Research Unit to distinguish between teliospores of T. indica and T. barclayana in stored grain, storage facilities, and transportation vehicles.
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