Avian granulosa cell prostaglandin secretion is regulated by transforming growth factor alpha and beta and does not control plasminogen activator activity during follicular development.
Li J. | Li M. | Lafrance M. | Tsang B.K.
The aim of the present study was to determine the role of transforming growth factor alpha (TGFalpha) and beta (TGFbeta) in the regulation of prostaglandin (PG) secretion, and the relationships between PG and plasminogen activator (PA) activity in hen granulosa cells during ovarian follicular development. Cells from the first (F1), third (F3), and fifth and sixth (F5-6) largest preovulatory follicles were cultured for up to 21 h in the presence of TGFalpha (0.1-10 ng/ml) and/or TGFbeta (4-20 ng/ml) or TGFalpha together with a cyclooxygenase inhibitor, indomethacin (0.05-0.5 micromolar). The release of PG into the incubation medium was determined by RIA. Cell-associated (PAc) and secreted (PAs) PA activities were measured by a fibrinolysis assay and characterized by zymography. Basal PGF secretion from F1, F3, and F5-6 cells was 2.2 +/- 0.3, 2.2 +/- 0.5, and 1.1 +/- 0.3 ng/micrograms DNA, respectively, and was higher than that of PGE. Basal total PA (PAc+PAs) activity from F1, F3, and F5-6 cells was 41 +/- 13, 261 +/- 68, and 958 +/- 268 X 10(3) cpm/micrograms DNA, respectively. TGFalpha stimulated PG secretion and PA activity in a dose-dependent manner. The TGFalpha-induced PA activity was predominantly associated with a molecular mass of 30-35 kDa, corresponding to that of urokinase PA. The stimulation of PG secretion by TGFa was maximal in F3 and F1 granulosa cells whereas PA activity in the presence of TGFalpha was highest in cells from F5-6 follicles. Dependent on the stage of follicular development, TGFbeta suppressed both basal and TGFalpha-stimulated PG secretion but enhanced the TGFalpha-induced PA activity. The inhibitory effect of TGFbeta on PG secretion induced by TGFalpha appeared to be more pronounced in the less differentiated cells. Indomethacin inhibited TGFalpha-induced PG secretion but had no effect on TGFalpha-induced PA activity.
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Wolters Kluwer